Salk Institute
Waitt Advanced Biophotonics Core

Instrumentation

The Biophotonics Center Core Facility exists to provide Salk Faculty with technical support for and logistical access to state-of-the-art imaging technologies to enable the next generation of biological breakthroughs at the Institute.

Available equipment for core use:

Super-Resolution Microscopy

  • Zeiss Elyra PS.1 Super-Resolution Microscope 
    This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and four color filtersets for both PAL-M and SR-SIM super-resolution imaging. It is equipped with two EM-CCD cameras, an Andor iXon3 897 512x512 35fps camera for PAL-M imaging in TIRF mode and an Andor iXon3 888 1024x1024 9 fps camera for 3D SR-SIM. The microscope also has a dedicated off-line processing workstation for the reconstruction of super-resolution imaging datasets.

Confocal Microscopy

  • Zeiss LSM 780 Side Port Laser Scanning Confocal Microscope
    This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal GAsP detector for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging.
  • Zeiss LSM 780 Rear Port Laser Scanning Confocal Microscope
    This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal GAsP detector for single-molecule sensitive full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging of living cells.
  • Zeiss LSM 710 Laser Scanning Confocal Microscope
    This inverted microscope is equipped with the following laser lines (405, 458, 488, 514, 561, 594 and 633nm). It is also equipped with a 34 element internal detector for full spectral imaging over the visible spectrum and a transmitted light detector for DIC imaging. It is also equipped with a Definite Focus module for extended time-lapse imaging of living cells.
  • Zeiss CSU Spinning Disk Confocal Microscope
    This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm) and a Yokogawa spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.
  • Yokogawa Cell Voyager 1000 Spinning Disk Confocal Microscope
    This live cell imaging system is equipped with the following laser lines (405, 488 and 561) and a Yokogawa dual-pinhole spinning disk scan head with an EM-CCD camera and a stage-top incubation system for the high-speed confocal imaging of living cells in culture.

Two-photon Microscopy

  • Olympus FV1000MPE Two-photon / CARS Microscope
    This upright microscope is equipped with the following single-photon laser lines (405, 440, 488, 514, 559, 633nm) as well as a Spectra-Physics MaiTai DeepSee infrared laser for two-photon excitation. It is equipped with 3 internal descanned detectors as well as 4 non-descanned two-photon fluorescence detectors and 2 forward scanning fluorescence detectors for Second Harmonic Generation (SHG) and Coherent Anti-Stokes Raman Microscopy (CARS).

TIRF Microscopy

  • Nikon Ti:E PFS TIRF Microscope
    This inverted microscope is equipped with the following laser lines (405, 488, 561 and 635nm), an Andor EM-CCD camera and the Nikon patented PerfectFocus module for TIRF imaging. It is also equipped with a stage-top incubation system for maintaining living cells in culture.

Laser Capture Microdissection

  • Zeiss PALM Microbeam V4 Laser Capture Micordissection System
    This inverted microscope is equipped with a UV (355nm) laser for the cutting, catapulting and robotic capture of fixed, frozen and living tissue. The system is equipped with advanced fluorescence and brightfield imaging capabilities as well as incubation to maintain cultured cells and tissue explants at physiologically relevant conditions.

Fluorescence Microscopy

  • Zeiss Axio Observer VivaTome Inverted Fluorescence Microscope
    This inverted microscope is an advanced fluorescence microscope equipped with a multi-color LED illumination system and a filtered white light source as well as a high-resolution cooled CCD camera for fluorescence detection. The system is also equipped with a VivaTome unit, which is an aperture correlation based optical sectioning device capable of acquiring confocal images at near video-rate. The system is also equipped with a color CCD camera for visualizing histological samples.
  • Zeiss StereoLumar V.12 Fluorescence Stereoscope
    This stereoscope is equipped with 6 color filtersets for fluorescence excitation as well as a cooled CCD camera for image acquisition. The system also has an white LED illuminator for the transillumination of specimens.
  • Olympus BX61WI Upright Fluorescence Microscope
    This upright microscope is equipped with a DG-5 fast wavelength switcher for fluorescence excitation and a fast filter wheel for fluorescence emission and an EM-CCD camera for high-speed single-molecule sensitive imaging.

Slide Scanning Microscopy

  • Olympus VS110 Virtual Slide Scanning Microscope
    This upright microscope is equipped with monochrome and color CCD cameras for the scanning of complete microscope slides with histological and fluorescence stains. Also equipped with a 100 slide loading capacity.
  • Metasystems Metafer Automated Slide Scanning and Analysis Microscope
    This upright microscope is equipped with monochrome and color CCD cameras for the scanning of complete microscope slides with histological or fluorescence stains. The scanning software is also capable of rare cell identification as well as karyotyping of and identification of metaphase spreads and telomere loss.

Electron Microscopy

  • Zeiss Libra 120 PLUS EF-TEM Transmission Electron Microscope
    This TEM microscope is equipped with a 2kx2k fiber-optically coupled bottom mount YAG CCD camera for image and montage acquisition, an in-column "Omega" energy filter for high contrast imaging in biological samples and a double tilt holder for tomography applications
  • Zeiss SIGMA VP Variable Pressure Scanning Electron Microscope
    SEM microscope is equipped with standard secondary electron, in-lens secondary electron and variable pressure (VP) detectors. System is also equipped with the ATLAS montage imaging module and a STEM detector as well as a cold-stage and the Shuttle and Find correlative microscopy navigation software and hardware.

Tissue Culture Facilities

  • Cell Culture Hoods
    The Core Facility has two laminar flow BSC cabinets for the sterile culture of mammialian cells.
  • CO2 incubators
    Four 37 deg C for maintaining mamammialian cells in culture.

Data Analysis and Storage

  • Data Storage
    The Core Facility has a 350TB data server for the storage of image data acquired using facility instrumentation.
  • Data Analysis Workstations
    Three dual quad-core processor 64bit workstations with 72GB of RAM equipped with Metamorph Premier, Imaris, MATLAB, ZEN 2010, AxioVision, ImageJ and Adobe Creative Suite software packages are available for image analysis and data processing and presentation requirements.

 


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